Various Ways to Explore Processivity of Eg5 Motor Protein cd.

4. CONCLUSIONS

Biochemical experiments confirm that Eg5 is non-processive. Processivity was obtained from gliding assays in the presence of methylcellulose (see section 3.2). From performed experiments the speed of Eg5 was measured and ATP and motor density dependences were analyzed. The highest speed of Eg5 is around 16 nm/s and with 1 μM ATP concentration microtubule movement was still possible to observe. These results can be used in future experiments to observe single Eg5 molecule using Optical Tweezers techniques.

Autor: Marta Mazur

BIOBLIOGRAPHY

[1] LOCKHART A., CROSS R. A., Kinetics and Motility of the Eg5 Microtubule Motor, Biochemistry, Vol. 35, 1996.
[2] PARMEGGIANI A., JULICHTER F., PELTIT L., PROST J., Detachment of molecular motors under targential loading, Europhysics Letters, 56 (4), pp. 603-609, 2001.
[3] HOWARD J., Mechanics of Motor Protein and Cytoskeleton, Sinauer, Sunderland, 2001.
[4.] DAWIDSON M. W., ABRAMOWITZ M., Optical Microscopy, chapter Differential Interference Contrast p. 33-35.